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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
13/01/2020 |
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Actualizado : |
01/06/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OBERTI, H.; DALLA RIZZA, M.; REYNO, R.; MURCHIO, S.; ALTIER, N.; ABREO, E. |
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Afiliación : |
HÉCTOR OBERTI RVAROLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Mycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827 |
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DOI : |
10.1080/00275514.2019.1694827 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19). |
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Contenido : |
Claviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before. |
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Palabras claves : |
ERGOT; FINGERPRINTING; HOST SPECIATION; PASPALUM; PHYLOGENY; STAGGER. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02438naa a2200277 a 4500
001 1060598
005 2020-06-01
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1080/00275514.2019.1694827$2DOI
100 1 $aOBERTI, H.
245 $aDiversity of Claviceps paspali reveals unknown lineages and unique alkaloid genotypes.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 25 Apr 2019 // Accepted 15 Nov 2019 // Published online: 07 Jan 2020. CONTACT: E. Abreo, eabreo@inia.org.uy. FUNDING: This work was supported by the National Institute of Agricultural Research (INIA) of Uruguay (grant numbers SA-24, BT-19).
520 $aClaviceps species affecting Paspalum spp. are a serious problem, as they infect forage grasses such as Paspalum dilatatum and P. plicatulum, producing the ergot disease. The ascomycete C. paspali is known to be the pathogen responsible for this disease in both grasses. This fungus produces alkaloids, including ergot alkaloids and indole-diterpenes, that have potent neurotropic activities in mammals. A total of 32 isolates from Uruguay were obtained from infected P. dilatatum and P. plicatulum. Isolates were phylogenetically identified using partial sequences of the genes coding for the second largest subunit of RNA polymerase subunit II (RPB2), translation elongation factor 1-? (TEF1), ?-tubulin (TUB2), and the nuc rDNA 28S subunit (28S). Isolates were also genotyped by randomly amplified polymorphic DNA (RAPD) and presence of genes within the ergot alkaloid (EAS) and indole-diterpene (IDT) biosynthetic gene clusters. This study represents the first genetic characterization of several isolates of C. paspali. The results from this study provide insight into the genetic and genotypic diversity of Claviceps paspali present in P. dilatatum and suggest that isolates from P. plicatulum could be considered an ecological subspecies or specialized variant of C. paspali. Some of these isolates show hypothetical alkaloid genotypes never reported before.
653 $aERGOT
653 $aFINGERPRINTING
653 $aHOST SPECIATION
653 $aPASPALUM
653 $aPHYLOGENY
653 $aSTAGGER
700 1 $aDALLA RIZZA, M.
700 1 $aREYNO, R.
700 1 $aMURCHIO, S.
700 1 $aALTIER, N.
700 1 $aABREO, E.
773 $tMycologia, 3 March 2020, Volume 112, Issue 2, Pages 230-243. Doi: https://doi.org/10.1080/00275514.2019.1694827
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Registro original : |
INIA Tacuarembó (TBO) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/09/2019 |
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Actualizado : |
10/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
VALLVERDÚ, C.; VILA, R.; LORENZO, D.; PAZ, D.; DELLACASSA, E.; DAVIES, P.; VILLAMIL, J.; TOMI, F.; CASANOVA, J.; CAÑIGUERAL, S. |
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Afiliación : |
CAROL VALLVERDÚ,, Universitat de Barcelona / Facultat de Farmàcia. Spain; ROSER VILA, Universitat de Barcelona / Facultat de Farmàcia. Spain.; DANIEL LORENZO, Universidad de la República (UdelaR)/ Facultad de Química.; DANIEL PAZ, Universidad de la República (UdelaR)/ Facultad de Química.; EDUARDO DELLACASSA, Universidad de la República (UdelaR)/ Facultad de Química.; PHILIP DAVIES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE MILTON VILLAMIL LUCAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELIX TOMI, Équipe de Chimie et Biomasse, Université de Corse, France.; JOSEPH CASANOVA, Équipe de Chimie et Biomasse, Université de Corse, France.; SALVADOR CAÑIGUERAL, Universitat de Barcelona / Facultat de Farmàcia. Spain. |
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Título : |
Composition of a new essential oil type of Lippia alba (Mill.) N.E. Brown from Uruguay. |
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Fecha de publicación : |
2001 |
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Fuente / Imprenta : |
Flavour and Fragrance Journal, 2001, Volume 16, Issue 5, Pages 356-359. |
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DOI : |
10.1002/ffj.1450 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 11 July 2000 / Revised 21 March 2001 / Accepted 24 March 2001. |
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Contenido : |
ABSTRACT.
The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC?MS and 13C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), ?-elemene (20.0%), ?-caryophyllene (8.8%) and ?-bourbonene (6.2%), whereas monoterpenes only reached 4% of the sample analysed.
Copyright © 2005 John Wiley & Sons, Ltd. |
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Palabras claves : |
Essential oil; Germacrene D; Lamiaceae; SALVIA GUARANITICA; Sesquiterpene hydrocarbons. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 01442naa a2200313 a 4500
001 1060218
005 2019-10-10
008 2001 bl uuuu u00u1 u #d
024 7 $a10.1002/ffj.1450$2DOI
100 1 $aVALLVERDÚ, C.
245 $aComposition of a new essential oil type of Lippia alba (Mill.) N.E. Brown from Uruguay.$h[electronic resource]
260 $c2001
500 $aArticle history: Received 11 July 2000 / Revised 21 March 2001 / Accepted 24 March 2001.
520 $aABSTRACT. The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC?MS and 13C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), ?-elemene (20.0%), ?-caryophyllene (8.8%) and ?-bourbonene (6.2%), whereas monoterpenes only reached 4% of the sample analysed. Copyright © 2005 John Wiley & Sons, Ltd.
653 $aEssential oil
653 $aGermacrene D
653 $aLamiaceae
653 $aSALVIA GUARANITICA
653 $aSesquiterpene hydrocarbons
700 1 $aVILA, R.
700 1 $aLORENZO, D.
700 1 $aPAZ, D.
700 1 $aDELLACASSA, E.
700 1 $aDAVIES, P.
700 1 $aVILLAMIL, J.
700 1 $aTOMI, F.
700 1 $aCASANOVA, J.
700 1 $aCAÑIGUERAL, S.
773 $tFlavour and Fragrance Journal, 2001, Volume 16, Issue 5, Pages 356-359.
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